mesenchymal stem cell basal medium  (ATCC)

Journal: iScience

Article Title: Fabrication of RADA32/Ngf_EE/MSCs composite hydrogel and its protective mechanism against radiation-induced ototoxicity

doi: 10.1016/j.isci.2026.115723

Figure Lengend Snippet: RNM composite gel mitigates radiation-induced damage in vitro (A) Viability of HEI-OC1 cells after different doses of radiation treatment, detected by CCK-8 assay. (B) Apoptosis of HEI-OC1 cells analyzed by flow cytometry after radiation exposure and subsequent transwell co-culture with RN gel, MSCs, or the RNM gel system. Data are represented as the mean ± SEM. ( N = 3, t test). (C) Distribution of γ-H2AX (red) in HEI-OC1 cells observed under a confocal microscope after radiation exposure and intervention with RN, MSCs, or RNM. Scale bars, 10 μm. (D, E, G, and H) mRNA expression levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) in mouse cochlear tissues evaluated by real-time qPCR after intratympanic injection of RN, MSCs, or RNM hydrogel following radiation exposure. Data are represented as the mean ± SEM ( N = 3, t test). (F) Intracellular ROS levels in HEI-OC1 cells monitored using the DCFH-DA fluorescent probe and flow cytometry after radiation exposure and intervention with RN, MSCs, or RNM. Data are represented as the mean ± SEM ( N = 3, t test). (I) mRNA expression levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) in HEI-OC1 cells detected by real-time qPCR after radiation exposure and intervention with RN, MSCs, or RNM. Data are represented as the mean ± SEM ( N = 3, t test). Significant differences between the groups are indicated by ∗ for p < 0.05, ∗∗ for p < 0.01, ∗∗∗ for p < 0.001, and ∗∗∗∗ for p < 0.0001.

Article Snippet: Mouse bone marrow-derived mesenchymal stem cells (MSCs) and the mouse monocyte/macrophage cell line RAW264.7 were purchased from Procell Life Science & Technology Co., Ltd.

Techniques: In Vitro, CCK-8 Assay, Flow Cytometry, Co-Culture Assay, Microscopy, Expressing, Injection

Journal: iScience

Article Title: Fabrication of RADA32/Ngf_EE/MSCs composite hydrogel and its protective mechanism against radiation-induced ototoxicity

doi: 10.1016/j.isci.2026.115723

Figure Lengend Snippet: In vivo protective effects of RNM composite gel (A) Schematic of the in vivo experimental timeline and anatomical images of the mouse cochlea under a dissection microscope. (a, oval window; b, cochlear labyrinth.). (B–G) ABR tests performed 2 days before radiation exposure and 3, 7, and 14 days after drug administration to assess hearing function in mice. (H) Left: Confocal microscopy images of the cochlear basilar membrane 14 days after radiation exposure and intratympanic injection of RN, MSCs, or RNM hydrogel, stained with FITC-phalloidin to label hair cells. Scale bars, 20 μm. Right: Quantitative comparison of outer hair cell (OHC) and inner hair cell (IHC) survival rates post-intervention. Data are represented as the mean ± SEM ( N = 3, t test). (I) H&E staining images of mouse cochlear tissues extracted 14 days after radiation exposure and intratympanic injection of RN, MSCs, or RNM hydrogel. Data are represented as the mean ± SEM ( N = 3, t test). Scale bars, 20 μm. Significant differences between the groups are indicated by ∗ for p < 0.05, ∗∗ for p < 0.01, ∗∗∗ for p < 0.001, and ∗∗∗∗ for p < 0.0001.

Article Snippet: Mouse bone marrow-derived mesenchymal stem cells (MSCs) and the mouse monocyte/macrophage cell line RAW264.7 were purchased from Procell Life Science & Technology Co., Ltd.

Techniques: In Vivo, Dissection, Microscopy, Confocal Microscopy, Membrane, Injection, Staining, Comparison

Journal: iScience

Article Title: Fabrication of RADA32/Ngf_EE/MSCs composite hydrogel and its protective mechanism against radiation-induced ototoxicity

doi: 10.1016/j.isci.2026.115723

Figure Lengend Snippet: Immunomodulatory mechanism of RNM composite gel (A and B) Flow cytometric analysis of CD86 and CD206 expression in RAW264.7 macrophages after irradiation and co-culture with RN, MSCs, or RNM composite gel in a transwell system (macrophages in lower chamber). Data are represented as the mean ± SEM ( N = 3, t test). (C) Immunofluorescence staining of F4/80 (red) on cochlear sections. Scale bars, 50 μm (a, spiral ganglion; b, basilar membrane; c, stria vascularis; d, spiral ligament). (D) Apoptosis of HEI-OC1 cells analyzed by flow cytometry after radiation exposure and intervention. Data are represented as the mean ± SEM ( N = 3, t test). (E) Expression level of p-p65, a key marker of NF-κB pathway activation, in macrophages after radiation exposure and drug intervention. Data are represented as the mean ± SEM ( N = 3, t test). Significant differences between the groups are indicated by ∗ for p < 0.05, ∗∗ for p < 0.01, ∗∗∗ for p < 0.001, and ∗∗∗∗ for p < 0.0001.

Article Snippet: Mouse bone marrow-derived mesenchymal stem cells (MSCs) and the mouse monocyte/macrophage cell line RAW264.7 were purchased from Procell Life Science & Technology Co., Ltd.

Techniques: Expressing, Irradiation, Co-Culture Assay, Immunofluorescence, Staining, Membrane, Flow Cytometry, Marker, Activation Assay

Journal: Regenerative Therapy

Article Title: Effects and mechanisms of synovial mesenchymal stem cell–derived extracellular vesicular LncRNA-SNHG14 on chondrocyte injury

doi: 10.1016/j.reth.2026.101122

Figure Lengend Snippet: A: Morphological characteristics of SMSCs observed under an inverted microscope. Representative images from three independent experiments are shown (n = 3) (scale bar = 100 μm). B: Flow cytometric analysis confirming the SMSC phenotype. The cells were highly positive for the mesenchymal marker CD29, and negative for the hematopoietic markers CD34 and CD45. This profile (CD29 + CD34–CD45–) is consistent with the ISCT criteria for mesenchymal stem cells (n = 3).

Article Snippet: Primary rat chondrocytes (Catalog No. CP-R087, 5 × 10 5 Cells/T25) and synovial mesenchymal stem cells (SMSC, Catalog No. CP-R304, 5 × 10 5 Cells/T25) were purchased from Wuhan Procell Life Science & Technology Co., Ltd.

Techniques: Inverted Microscopy, Marker

Journal: Regenerative Therapy

Article Title: Engineering cardiac regeneration using stem cells: Cellular sources, differentiation signatures, targeted delivery, and functional recovery

doi: 10.1016/j.reth.2026.101120

Figure Lengend Snippet: Pluripotency and differentiating capacity of embryonic stem cells (ESCs). ESCs are created from the inner cell mass (ICM) of the blastocyst stage. They can develop into all three germ layers: the ectoderm (which later gives rise to the brain, skin, and eyes), the endoderm (which gives rise to the lungs, liver, and gut), and the mesoderm (which gives rise to the bones, blood, and muscles). ESCs undergo in vitro differentiation using specific protocols, forming embryoid bodies that mimic early-stage embryogenesis. They may also be implanted in vivo, where they can assist in tissue regeneration. Still, they may, in rare cases, develop into teratomas: complex tumors that contain tissues from all three germ layers. ESCs may also assist in repairing organs, although they may be unregulated and pose risks such as teratocarcinoma.

Article Snippet: Embryonic Stem Cells (ESCs) , - Regeneration of damaged myocardium - replacement of cardiomyocytes , Differentiation into functional cardiomyocytes , Geron Corporation studies (preclinical models) [ ] .

Techniques: Muscles, In Vitro, In Vivo

Journal: Materials Today Bio

Article Title: Targeted antibacterial and mesenchymal stem cell-modulatory hydrogel for periodontitis treatment

doi: 10.1016/j.mtbio.2026.103043

Figure Lengend Snippet: Schematic diagram of the ACP@Z@C hydrogel for periodontitis treatment. The BA-modified CC hydrogel for the delivery of CAPE-loading MOF, which accomplishes the targeted and controlled release of ZIF-8@CAPE in oral microenvironment. The released ZIF-8@CAPE interferes with multiple periodontitis-driven factors, including anti-bacteria, ROS-scavenging, and anti-inflammation. These potency transforms into periodontal tissue regeneration via rescuing the impaired osteogenic differentiation of MSCs.

Article Snippet: Rat bone marrow mesenchymal stem cells (MSCs) was purchased from Procell (Wuhan, China).

Techniques: Modification, Bacteria

Journal: Materials Today Bio

Article Title: Targeted antibacterial and mesenchymal stem cell-modulatory hydrogel for periodontitis treatment

doi: 10.1016/j.mtbio.2026.103043

Figure Lengend Snippet: Inhibiting inflammatory factor by adjusting mitochondrial dysfunction via SIRT1/p-AMPK/PGC-1α pathway. (A) Schematic illustration of the molecular mechanism by which Z@C regulates mitochondrial dysfunction and suppresses inflammatory factor production in MSCs. (B) Representative western blot bands and quantitative analysis of (C) SIRT1, (D) p-AMPK, (E) PGC-1α, (F) NLRP3, and (G) Pro-Caspase-1 protein expression. Data were presented as mean ± SD, n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. Expression levels of (H) SIRT1, (I) PGC-1α, (J) NLRP3, (K) IL-1β, (L) IL-6, and (M) TNF-α following Z@C treatment. Data were presented as mean ± SD, n = 5, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. (N) Immunofluorescence staining of SIRT1 expression in MSCs following different groups and (O) quantitative analysis. Data were presented as mean ± SD, n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: Rat bone marrow mesenchymal stem cells (MSCs) was purchased from Procell (Wuhan, China).

Techniques: Western Blot, Expressing, Immunofluorescence, Staining